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(A) Experimental design of the anti-IL-1β antibody study in the FOP mouse model. Mice received weekly intraperitoneal injections beginning at P27 (day 0) of either vehicle (saline), <t>IgG2A</t> isotype control antibody, or the anti-IL-1β monoclonal antibody 01BSUR (10 mg/kg). Trauma-induced HO was generated using a ball-drop injury model applied to the right hind limb at P28. HO formation was quantified by µCT at day 21. Created with Biorender.com. (B) Quantification of trauma-induced HO volume measured by µCT. Each point represents an individual mouse, and boxes show group distributions. Treatment with the anti-IL-1β antibody 01BSUR significantly reduced HO volume compared with both vehicle-treated mice (p = 0.0087) and IgG2A isotype controls (p = 0.0095). (C) Representative three-dimensional µCT reconstructions of skeletal structures from each treatment group. Areas of HO formation are highlighted. Extensive HO is observed in vehicle- and IgG2A-treated mice, whereas mice treated with the anti-IL-1β antibody show markedly reduced HO at the injury site. Representative images were chosen based on the mean HO volume of each group.
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(A) Experimental design of the anti-IL-1β antibody study in the FOP mouse model. Mice received weekly intraperitoneal injections beginning at P27 (day 0) of either vehicle (saline), IgG2A isotype control antibody, or the anti-IL-1β monoclonal antibody 01BSUR (10 mg/kg). Trauma-induced HO was generated using a ball-drop injury model applied to the right hind limb at P28. HO formation was quantified by µCT at day 21. Created with Biorender.com. (B) Quantification of trauma-induced HO volume measured by µCT. Each point represents an individual mouse, and boxes show group distributions. Treatment with the anti-IL-1β antibody 01BSUR significantly reduced HO volume compared with both vehicle-treated mice (p = 0.0087) and IgG2A isotype controls (p = 0.0095). (C) Representative three-dimensional µCT reconstructions of skeletal structures from each treatment group. Areas of HO formation are highlighted. Extensive HO is observed in vehicle- and IgG2A-treated mice, whereas mice treated with the anti-IL-1β antibody show markedly reduced HO at the injury site. Representative images were chosen based on the mean HO volume of each group.

Journal: bioRxiv

Article Title: Gut microbiome-dependent IL-1 signaling is a mediator of ACVR1 R206H -driven heterotopic ossification

doi: 10.64898/2026.04.05.716562

Figure Lengend Snippet: (A) Experimental design of the anti-IL-1β antibody study in the FOP mouse model. Mice received weekly intraperitoneal injections beginning at P27 (day 0) of either vehicle (saline), IgG2A isotype control antibody, or the anti-IL-1β monoclonal antibody 01BSUR (10 mg/kg). Trauma-induced HO was generated using a ball-drop injury model applied to the right hind limb at P28. HO formation was quantified by µCT at day 21. Created with Biorender.com. (B) Quantification of trauma-induced HO volume measured by µCT. Each point represents an individual mouse, and boxes show group distributions. Treatment with the anti-IL-1β antibody 01BSUR significantly reduced HO volume compared with both vehicle-treated mice (p = 0.0087) and IgG2A isotype controls (p = 0.0095). (C) Representative three-dimensional µCT reconstructions of skeletal structures from each treatment group. Areas of HO formation are highlighted. Extensive HO is observed in vehicle- and IgG2A-treated mice, whereas mice treated with the anti-IL-1β antibody show markedly reduced HO at the injury site. Representative images were chosen based on the mean HO volume of each group.

Article Snippet: IgG2A isotype antibody from R&D Systems (catalog no. MAB0031) was used as a control.

Techniques: Saline, Control, Generated